Enzyme Research on Nuclease
By Onyx Coates
Nuclease:The nucleases substrate is DNA/RNA https://answers.yahoo.com/question/index?qid=20110...
A product of nucleases is mung bean nuclease https://www.neb.com/products/dna-modifying-enzymes...
Source of enzyme: comes from the blood http://www.genetik.uni-koeln.de/groups/Kemper/nucl...
Description of reaction and why its important:
1. Degradation of contaminating DNA after RNA isolation,
2. "Clean-up" of RNA prior to RT-PCR and after in vitro transcription,
3. Identification of protein binding sequences on DNA (DNase I footprinting),
4. Prevention of clumping when handling cultured cells, and
5. Creation of a fragmented library of DNA sequences for in vitro recombination reactions.
Its important to help make DNA and RNA
Optimum temperature and pH of the enzyme:
The optimum stability of the enzyme is at pH 5 - 5.5, with rapid inactivation at pH 8.5 at 30 °C. - See more at: http://www.sigmaaldrich.com/life-science/metabolo...