Egg Cell Osmosis Lab

By: Chris John


Objective

To perform an experiment consisting of an egg and other substances to observe the reactions caused by the principles of the diffusion of molecules and osmosis.

Predictions

Day 1- Vinegar Test

Our prediction for the vinegar test is that the egg will change in size and the shell of the egg will become thinner.

Day 2- Syrup Test

Our prediction for the syrup test is that the egg will turn brown (not including shell since its already gone).

Day 3-  Colored Water Test

Or prediction for the colored water test is that the egg will soak in all the dye and water, changing the color of the egg.

Day 4- Penetrating Egg Test

Our prediction for the penetrating egg test is that the egg will pop and the yolk will turn blue. We also expect the fluid that the egg has absorbed to be let free.


Materials

  • 1 graduated cylinder
  • 1 egg
  • 1 cup
  • 100 ml. vinegar
  • 100 ml. syrup
  • 100 ml. water
  • approximately 5 drops of food coloring
  • 1 measuring tape
  • 1 piece of aluminum foil
  • 1 sharpie
  • 1 triple-beam balance

Procedures

Vinegar and Egg Procedure

1. Measure length of egg in cm. longitudinal and transverse.

2. Measure the mass of the egg.

2. With the egg in the cup, pour vinegar in the cup (100 ml.) until it covers all of the egg. Place the foil over the cup.

3. Leave egg covered in the cup for three days.


Syrup and Egg Procedure

1. Take the egg out of the cup and record new measurements.

2. Measure the volume of the vinegar and the difference between the original amount.

3. Rinse out the vinegar left behind.

4. Pour 100 mL of the syrup and cover the egg with foil.

5. Leave egg covered in foil for one day.

Colored Water and Egg Procedure

1. Take the egg out of the cup and record new measurements.

2. Measure the volume of the syrup and the difference between the original amount.

3. Clean out cup and pour in 100 mL of water.

4. Place no more than five drops of food coloring in the cup along with the egg.

5. Leave the egg in the cup for one day.

*We used three drops of blue dye and two drops of purple dye.

Popping the Egg Procedure

1. Take the egg out of the water, and take its measurements.

2. Measure the volume of the water and the difference between the original amount.

3. Rinse out the cup filled with water.

4. Put the egg back in the cup and use a toothpick to open the egg.

5. Record measurements of all parts needed to be measured in cm.

Analysis

Vinegar Analysis

After performing the test of adding an egg into vinegar solution for three days, I can infer that the vinegar was a hypotonic solution, because the vinegar in the cup was less than we had before waiting. The egg was considerably larger after three days, going from 17 cm. longitudinal and 14cm. transverse to 18 cm. longitudinal and 16 cm. transverse. After comparing our measurements, we realized that the amount of vinegar in the cup was less, concluding that the relationship was hypotonic, meaning that there was more water (or vinegar in this case) than solute.

Syrup Analysis

After performing the test of adding an egg into syrup solution for a day, I can infer that the syrup was a hypertonic solution, because the syrup in the cup was more that we had before waiting. The egg didn't change much in size, but the mass of the egg changed very much, changing from 94.3 g. to 78.1 g. after adding the syrup solution in the cup for a day. The water moved out of the egg, resulting in more syrup outside of the egg than before, or in other words there was more solute than solvent. Plasmolysis occurred, therefore resulting in a hypertonic situation.

Colored Water Analysis

After performing the test of adding an egg into water solution for a day, I can infer that the water was a isotonic situation, because after waiting a day, the measurements haven't drastically changed. The egg did slightly grow larger in size, but overall, the egg stayed around the same area as when the syrup was added. The reason the egg did grow despite it being an isotonic situation was because osmosis still occurs during isotonic situations, just equally on both sides to maintain equilibrium.

Comparisons

After observing all the tests, we realized that the egg was bigger in the water situation, because after the diffusion that occurred after the syrup, the water was all absorbed again in the water. Cytolysis did not occur, because the egg was not given large enough amounts of each substance. The water was blue after we popped the egg (we added 3 drops of blue dye and 2 drops of purple dye) and we can infer that all that color came from the water that was absorbed.Throughout this whole lab, the semi-permeable membrane has let substances come and let out, but in the end, the egg was considerably bigger.

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